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Wonder Woman (1942): Saturnian slavers have been abducting humans for some years to sell to the Empire as slave labor, though some people were abducted by cruel humans like Hypnota and sold to the slavers.Sterling Roquette and a couple other Cadmus personnel get abducted and enslaved by the extraterrestrial slaver Kossak while Cadmus is trying to decipher the workings of a small space craft revealed to have been stolen from Kossak by an escaping slave before it crashed on earth. Invasion!: The Dominators abduct humans to experiment with the meta-gene, and the shapeshifting Durlans abduct humans to imitate and replace them.His father survived captivity and eventually became the Space Pirate Corsair. The backstory of Cyclops of the X-Men has his parents being abducted by Shi'Ar Emperor D'Ken, who seemingly just picked them at random because he's nuts.They normally keep their victims alive just in case they might be useful in the future. The Skrulls, a shapeshifting alien race who are primarily Fantastic Four villains, are in the habit of abducting humans in order to replace them for espionage purposes (at first because Earth was strategically located in their Forever War with the rival Kree Empire, later because they've managed to make enemies of Earth's many superheroes).If the aliens' interest is scientific research, expect them to be terrible at it: No Control Group, no reliable tranquilizers (because how else could abductees keep waking up too soon, so they can see they're in a spaceship?), and no acknowledgment that it's indiscreet to keep abducting idiots who'll rush to spill their story to the tabloids. Sometimes, if the alien is benign, the human will witness something that makes the abduction worth the trouble. Or they have been forced to be The Chosen One. And even a benign alien can cause an abduction, and cause problems, if Time Travel in either direction happens. They were most certainly probed and violated though. They may have a little tracker planted in them. They may have had a complete physical, possibly without anesthesia.
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Humans returned from alien abductions usually have had bad things happen to them. Alien abductions do get witnessed, but the witnesses are almost always portrayed as a bit mad even when the alien abduction is real in canon. The alien generally leaves no trace of the human until it's done with it. When an alien from another planet, whether one of the Little Green Men or one of The Greys or whatever, kidnaps a human (often by way of Tractor Beam).